1. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM SpectraMax M5 Microplate Reader: Read type = endpoint, Read Mode = luminescence with 1000 ms integration time, Wavelengths = All, Automix = off, Auto calibration = on, Settling time = off, Autoread = off. The dual monochromator optics allow the. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. 5 Dual-luciferase reporter assay. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. The following document is. The SpectraMax M2 and M2e are versatile and reliable. 7 1. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. The SpectraMax i3x Microplate Reader possesses the five detection modes preferred by the majority of users: The SpectroMax i3x Microplate Reader is the standard for UV/ Visible multimode reader absorbance, providing ultrafast, full spectral range dectection for cuvettes as well as 96- and 384-well microplates. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. IMAP FP Settings in SoftMax Pro M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. SpectraMax® M5. 0. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. both cuvette-based and microplate reader applications on the same instrument. Pick the best speed, sensitivity, and price for your needs. The SpectraMax® ABS Plus Microplate Reader can run . However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. Path Check Pathlength Measurement SpectraMax Spectrofluorometers. Add to Cart. When a feature or capability applies to only certain readers, this exception is noted. This application note describes how to. One vial of Thaw-and-Use TIGIT Effector Cells was thawed and resuspended in cell recovery. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The spectrophotometric. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. Flurescence intensities were read using a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 59 6. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Tunable wavelength selection / monochromator. 313 reference gain check fail. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. SpectraMax M5e Multi-Mode Microplate Reader. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!The microplate readers in SpectraMax M5 series can also provide high-throughput capabilities with its automated microplate handling and storage systems. 4. For the Live/Dead assay, they were treated. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. com. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Microplate Readers are modular and . The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. At the end of this period, cells were exposed to FA (1 mM) for 1 h. The plate reader can measure multiple readouts, including UV and visible absorbance. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. Make sure the microplate is flat against. Label: provides information about the reader, such as line voltage rating, caution-ary information, serial number, etc. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. 73. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuide SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. When the user applies a set temperature of 37°C, the top is heated to 37. SpectraTest Validation Plates form Molecular Devices such as ABS1 absorbance, FL1 fluorescence & LM1 luminescence validation plates are the valuable tools for verifying that absorbance, fluorescence, and luminescence-capable microplate readers are operating correctly for GLP and GMP laboratories. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Western blot analyses. Physical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. The concentration of antibodies was determined by using a spectrophotometer from Puxi General Instrument Co. 1 & 7. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. Molecular Devices Corporation. Figure 1. We would like to show you a description here but the site won’t allow us. book Page 1 Wednesday, July 7, 2010 2:09 PM The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 1. Key applications: ELISA, nucleic acid, endotoxin testing. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Offer Ends In: 44 Days 10: 22: 56. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Table of Contents. Cell viability was assayed by addition of BacTiter-Glo ™ bioluminescence reagent according to the manufacturer’s protocol (Promega) and luminescence measured using a Spectramax M5 microplate reader (Molecular Devices). 314 low lamp level warning. Introduction. In brief, RAW 264. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Then, the fluorescence change rates (F 0 − F)/F 0 were calculated, and the selectivity histogram. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 1–0. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. metrically at 540 nm using a microplate reader (SpectraMax 384 Plus, Molecular Devices Corp. The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. We would like to show you a description here but the site won’t allow us. 0 . TNF standard curve. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. SpectraMax M Series multi-mode microplates are configurable readers with triple. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. 00 USD. 0 out of 5 $ 3,799. Protocol Name Version 6. Description. The day before assay, assay buffer was prepared as described in the product technical manual. The assay window spanned four orders of magnitude. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Fluorescence from the Alamar blue® assay was detected by SpectraMax M5 microplate reader (Molecular Device Inc. The absorbance value of each reaction solution was measured at 405 nm by a Molecular Device SpectraMax M5 microplate reader (Sunnyvale, CA, USA). The spectrophotometric. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Examples: “Press carefully to engage,” “ Do not. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. Materials. Then, 150 μL of dimethyl sulfoxide was added to each well and shaken gently for 10 min. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Please refer to the Obsolete Microplate Reader Models for more information. The Spectr. After being incubated in the dark at 60 ℃ overnight, the supernatants were centrifuged and optical densities were measured at 750, 665, 652 and 470 nm using the SpectraMax M5 microplate reader (Molecular Devices,. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. FlexStation™ II. SpectraMax® ABS. 0 out of 5 $ 16,499. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. Urea-induced unfolding of rA1AT-WT and the Q9N mutant was measured using a SpectraMax. Fifteen different automated tests are provided. The dual monochromator optics allow the widest range of applications to be utilized for bior. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Choose from a. 5 Compatible microplate readers FP Plate Reader SpectraMax® M5/M5e Multi-Mode Microplate ReaderSpectraMax M5 Multi-Mode Microplate Reader; Method. Lower limits of detection for ATP determined using the ATPlite . Background fluorescence has been subtracted. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. com. Designed to provide the simplicity, flexibility and power required for advanced data analysis. Wide range of applications The VersaMax reader covers. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-,. Detection cartridge modularity allows for easy addition of new read modes within minutes without. com A. Standard SDS-PAGE was performed with a 10% (w/v) separating gel and a 4% stacking gel. SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. B: Selection of Excitation Wavelength. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Linear, Orbital Slow, and Orbital Fast. KEY FEATURES • Five. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 0 out of 5 $ 2,499 $ 1,999. A: Microplate Preparation. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. FlexStation™ I. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Microplate Readers Biotek Epoch Microplate. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. The drawing shows a standard microplate of 15 mm height covered with a lid. Supplier: Molecular Devices. Amino acids assay. The SpectraMax L Microplate Reader was set up for endpoint reading with an inte-gration time of 1 second or 0. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Keywords. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. The readings with excitation at 360 nm and emission at 450 nm represented the amount of. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. 95. 0 μL) instead of the sample solution was applied as control group, and. VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® Plus 384 Absorbance Microplate Reader; SpectraMax® L (software version 5. Microplate Readers Biotek Synergy H1 Hybrid Microplate. Cuvette port functionality can also be controlled using SoftMax Pro Software. 04 to 3. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Bioz Stars score: 86/100, based on 1 PubMed citations. Add to Cart. Microplate Readers are modular and . The Multiskan Sky microplate reader is an absorbance plate reader. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. The chamber contains springs that position the cuvette in the proper alignment for a read. " The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The guide also covers the software features, the specifications, and the accessories of the systems. Molecular Devices SpectraMax M5 Multimode Microplate Reader. Our absorbance plate readers feature our PathCheck Sensor. 40266 Email: drugdiscoverytech@lifetech. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. After 3 min of reaction, the fluorescence emission spectra of the mixed solutions were recorded using a multifunctional microplate reader (SpectraMax M5, Molecular Devices, USA) at the excitation wavelength (λ ex) of 485 nm. Lift up the lid over the chamber to insert or remove a cuvette. Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. book Page 7 Wednesday, July 7, 2010 2:09 PM. FlexStation™ III. The absorbance values at 450 nm were determined by the SpectraMax M5 microplate reader. Here the delta mP was 336, and Z’ factor was 0. The cells were then treated to induce apoptosis. 05% Tween 20 using a Nunc ELISA plate washer. ATP standard curves read on SpectraMax microplate readers. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. SDS-PAGE Assay. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. 6-384 (96- and 384-well with pipetting) 6-384. Nontreated cells in DMEM medium were used as a control. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter. The dual monochromator optics allow the widest. The raw data from each individual experiment were normalized to vehicle-treated HK-2. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Microplate Capacity. 1. Microplate Readers Biotek ELx808 Microplate Reader Absorbance. Recommended Optics Wavelength (nm) Wavelength. Expanded wavelength coverage. 6: bioNova científica, s. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 6-384. You should get comparable results from top and bottom reads using EC50's to compare reads. Published Dec 16, 2020 | Updated Dec 16, 2020. We would like to show you a description here but the site won’t allow us. SoftMax Pro Software has been integrated by many leading robotics andFor readers without a cuvette port, simply check the box next to ‘PathCheck’. l. RAW 264. After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. When a feature or capability applies to only certain readers, this exception is noted. 0 μL) instead of the enzyme solution was used as the sample blank group. 2. Microplate Readers Include the M3, M4, M5, and M5. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Conclusions We report a peptide-induced super-assembly of enzyme-containing MOFs for programmed enzyme. Molecular Devices Spectramax M5 Microplate Reader. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. Standard features include a. Wide range of applications. Equal amount of proteins were loaded and resolved using denaturing gel and analyzed by western blot using antibodies recognizing Akt, rS6, mOTR and. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. Western blotting analysis. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the signal is constant. 7°C (gradient heating design). Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. spectramax m5 multi. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. 1step assay with SpectraMax microplate readers. 303 unable to cal dark current. book Page 7 Wednesday, July 7, 2010 2:09 PM. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. 3 mg. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. Self Service Available. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Figure 1. 1 second. 1 is GxP edition only; Protocols for the ABS1 Validation Plate. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. • Tel. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Cuvette port functionality can also be controlled using SoftMax Pro Software. Also refer to this Multimode Reader Comparison Chart . The SpectraMax 190 reader can be integrated with Molecular . view more . SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. 4. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. edu) to be added as a trained user in iLab. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. E Models. 3. Between steps, reactions were rinsed 3 times with TBS + 0. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 8 µg pNF-κB-After 3 h of incubation at 37 °C, the medium was gently removed, and a 150-μL aliquot of DMSO was added to each well. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Western blotting assay. 0 out of 5 $ 17,999 $ 16,995. 4 0. 312 gain calibration failed. Self Service Available. 1. Spectramax M5 Microplate Reader, supplied by Danaher Inc, used in various techniques. Bottom-reading will also give you higher RFU's (and the appearance of high sensitivity) because the polystyrene will fluoresce a bit. Incubation. Cell viability was quantified by measuring the absorbance of the solution at 570 nm using a SpectraMax® M5 microplate reader (Molecular Devices). The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Compatible automation solutions for SpectraMax Readers. Maximum signal with lowest background. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Figure 1. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. 0 out of 5 $ 17,999 $ 16,995. 1. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. In the M2, M5 and Plus. 7°C and the bottom to 36. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). Microplate Readers include the M3, M4, M5, and M5e models. This reader's dual monochromators for. Ltd. For biological applications involving small samples, such as purificationThe guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 2. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. SpectraMax iD5 Multi-Mode Microplate Reader. flexibility of monochromator-based systems. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. TR-FRET assay. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. 00. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. 00. Step 1. Five preferred detection modes. The absorbance values at 450 and 570 nm were determined using the SpectraMax M5 microplate reader (Molecular Devices, Silicon Valley, CA, United States). STAKMAX. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. Read More. SpectraMax Glo Steady-Luc Reporter Assay Kit. Molecular Devices Spectramax M5 Microplate Reader. Qualify the luminescence performance of the SpectraMax . The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. 49 2. 2. , USA). In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Trilevel commercial control reagents (StanbioThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. 95. 67. Fluorescence was measured (Ex 530/Em 590) in a SpectraMax M5 microplate reader. Examples: “Press carefully to engage,” “ Do not. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . 305 reference level saturation. ) injection of 3 mLThe fluorescence intensity was detected by using a SpectraMax M5 microplate reader from Molecular Devices Co. The absorbance was measured at 490 nm using a SpectraMax® M5 microplate reader (Molecular Devices, USA). 1. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. 5 87.